Publications

Biology of vascular mural cells.
Development (Cambridge, England). 2023 Aug 15;150(16) Epub 2023 Aug 28; Authors: Siekmann AF

Temporally and regionally distinct morphogenetic processes govern zebrafish caudal fin blood vessel network expansion.
Development (Cambridge, England). 2023 Apr 1;150(7) Epub 2023 Apr 11; Authors: Leonard EV, Hasan SS, Siekmann AF

Zebrafish mutants in vegfab can affect endothelial cell proliferation without altering ERK phosphorylation and are phenocopied by loss of PI3K signaling.
Developmental biology. 2022 Jun;486:26-43 Epub 2022 Mar 23; Authors: Lange M, Ohnesorge N, Hoffmann D, Rocha SF, Benedito R, Siekmann AF

Regenerating vascular mural cells in zebrafish fin blood vessels are not derived from pre-existing mural cells and differentially require Pdgfrb signalling for their development.
Development (Cambridge, England). 2022 Apr 1;149(7) Epub 2022 Apr 5; Authors: Leonard EV, Figueroa RJ, Bussmann J, Lawson ND, Amigo JD, Siekmann AF

Shear stress switches the association of endothelial enhancers from ETV/ETS to KLF transcription factor binding sites.
Scientific reports. 2022 Mar 21;12(1):4795; Authors: Tsaryk R, Yucel N, Leonard EV, Diaz N, Bondareva O, Odenthal-Schnittler M, Arany Z, Vaquerizas JM, Schnittler H, Siekmann AF

Temperature-responsive optogenetic probes of cell signaling.
Nature chemical biology. 2022 Feb;18(2):152-160 Epub 2021 Dec 22; Authors: Benman W, Berlew EE, Deng H, Parker C, Kuznetsov IA, Lim B, Siekmann AF, Chow BY, Bugaj LJ

Transcriptional regulatory network controlling the ontogeny of hematopoietic stem cells.
Genes & development. 2020 Jul 1;34(13-14):950-964 Epub 2020 Jun 4; Authors: Gao P, Chen C, Howell ED, Li Y, Tober J, Uzun Y, He B, Gao L, Zhu Q, Siekmann AF, Speck NA, Tan K

Identification of atheroprone shear stress responsive regulatory elements in endothelial cells.
Cardiovascular research. 2019 Aug 1;115(10):1487-1499; Authors: Bondareva O, Tsaryk R, Bojovic V, Odenthal-Schnittler M, Siekmann AF, Schnittler HJ

Advantages and Challenges of Cardiovascular and Lymphatic Studies in Zebrafish Research.
Frontiers in cell and developmental biology. 2019;7:89 Epub 2019 May 28; Authors: Santoro MM, Beltrame M, Panáková D, Siekmann AF, Tiso N, Venero Galanternik M, Jung HM, Weinstein BM

Veins and Arteries Build Hierarchical Branching Patterns Differently: Bottom-Up versus Top-Down.
BioEssays : news and reviews in molecular, cellular and developmental biology. 2019 Mar;41(3):e1800198; Authors: Red-Horse K, Siekmann AF

Future directions for therapeutic strategies in post-ischaemic vascularization: a position paper from European Society of Cardiology Working Group on Atherosclerosis and Vascular Biology.
Cardiovascular research. 2018 Sep 1;114(11):1411-1421; Authors: Caporali A, Bäck M, Daemen MJ, Hoefer IE, Jones EA, Lutgens E, Matter CM, Bochaton-Piallat ML, Siekmann AF, Sluimer JC, Steffens S, Tuñón J, Vindis C, Wentzel JJ, Ylä-Herttuala S, Evans PC

Coronary artery development, one cell at a time.
Nature. 2018 Jul;559(7714):335-336; Authors: Siekmann AF

Endothelial cell biology of Endoglin in hereditary hemorrhagic telangiectasia.
Current opinion in hematology. 2018 May;25(3):237-244; Authors: Sugden WW, Siekmann AF

Genetic dissection of endothelial transcriptional activity of zebrafish aryl hydrocarbon receptors (AHRs).
PloS one. 2017;12(8):e0183433 Epub 2017 Aug 17; Authors: Sugden WW, Leonardo-Mendonça RC, Acuña-Castroviejo D, Siekmann AF

Endothelial Notch signalling limits angiogenesis via control of artery formation.
Nature cell biology. 2017 Aug;19(8):928-940 Epub 2017 Jul 17; Authors: Hasan SS, Tsaryk R, Lange M, Wisniewski L, Moore JC, Lawson ND, Wojciechowska K, Schnittler H, Siekmann AF

Endoglin controls blood vessel diameter through endothelial cell shape changes in response to haemodynamic cues.
Nature cell biology. 2017 Jun;19(6):653-665 Epub 2017 May 22; Authors: Sugden WW, Meissner R, Aegerter-Wilmsen T, Tsaryk R, Leonard EV, Bussmann J, Hamm MJ, Herzog W, Jin Y, Jakobsson L, Denz C, Siekmann AF

Reprimo tissue-specific expression pattern is conserved between zebrafish and human.
PloS one. 2017;12(5):e0178274 Epub 2017 May 31; Authors: Figueroa RJ, Carrasco-Avino G, Wichmann IA, Lange M, Owen GI, Siekmann AF, Corvalán AH, Opazo JC, Amigo JD

MicroRNA 139-5p coordinates APLNR-CXCR4 crosstalk during vascular maturation.
Nature communications. 2016 Apr 12;7:11268; Authors: Papangeli I, Kim J, Maier I, Park S, Lee A, Kang Y, Tanaka K, Khan OF, Ju H, Kojima Y, Red-Horse K, Anderson DG, Siekmann AF, Chun HJ

Intubation-based anesthesia for long-term time-lapse imaging of adult zebrafish.
Nature protocols. 2015 Dec;10(12):2064-73 Epub 2015 Nov 19; Authors: Xu C, Volkery S, Siekmann AF

The same but different: signaling pathways in control of endothelial cell migration.
Current opinion in cell biology. 2015 Oct;36:86-92 Epub 2015 Aug 1; Authors: Hasan SS, Siekmann AF

Chemokine-guided angiogenesis directs coronary vasculature formation in zebrafish.
Developmental cell. 2015 May 26;33(4):442-54; Authors: Harrison MR, Bussmann J, Huang Y, Zhao L, Osorio A, Burns CG, Burns CE, Sucov HM, Siekmann AF, Lien CL

Arteries are formed by vein-derived endothelial tip cells.
Nature communications. 2014 Dec 15;5:5758; Authors: Xu C, Hasan SS, Schmidt I, Rocha SF, Pitulescu ME, Bussmann J, Meyen D, Raz E, Adams RH, Siekmann AF

Distinct Notch signaling outputs pattern the developing arterial system.
Development (Cambridge, England). 2014 Apr;141(7):1544-52 Epub 2014 Mar 5; Authors: Quillien A, Moore JC, Shin M, Siekmann AF, Smith T, Pan L, Moens CB, Parsons MJ, Lawson ND

Blood flow changes coincide with cellular rearrangements during blood vessel pruning in zebrafish embryos.
PloS one. 2013;8(10):e75060 Epub 2013 Oct 11; Authors: Kochhan E, Lenard A, Ellertsdottir E, Herwig L, Affolter M, Belting HG, Siekmann AF

The tip cell concept 10 years after: new players tune in for a common theme.
Experimental cell research. 2013 May 15;319(9):1255-63 Epub 2013 Feb 16; Authors: Siekmann AF, Affolter M, Belting HG

Zebrafish as a model to study chemokine function.
Methods in molecular biology (Clifton, N.J.). 2013;1013:145-59; Authors: Kochhan E, Siekmann AF

Chemokine signaling directs trunk lymphatic network formation along the preexisting blood vasculature.
Developmental cell. 2012 Apr 17;22(4):824-36; Authors: Cha YR, Fujita M, Butler M, Isogai S, Kochhan E, Siekmann AF, Weinstein BM

Evaluation and application of modularly assembled zinc-finger nucleases in zebrafish.
Development (Cambridge, England). 2011 Oct;138(20):4555-64; Authors: Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ, Look AT, Epstein JA, Lawson ND, Wolfe SA

The role of chemokines and their receptors in angiogenesis.
Cellular and molecular life sciences : CMLS. 2011 Sep;68(17):2811-30 Epub 2011 Apr 9; Authors: Kiefer F, Siekmann AF

Arterial-venous network formation during brain vascularization involves hemodynamic regulation of chemokine signaling.
Development (Cambridge, England). 2011 May;138(9):1717-26 Epub 2011 Mar 23; Authors: Bussmann J, Wolfe SA, Siekmann AF

Reiterative use of the notch signal during zebrafish intrahepatic biliary development.
Developmental dynamics : an official publication of the American Association of Anatomists. 2010 Mar;239(3):855-64; Authors: Lorent K, Moore JC, Siekmann AF, Lawson N, Pack M

Notch-responsive cells initiate the secondary transition in larval zebrafish pancreas.
Mechanisms of development. 2009 Oct;126(10):898-912 Epub 2009 Jul 10; Authors: Parsons MJ, Pisharath H, Yusuff S, Moore JC, Siekmann AF, Lawson N, Leach SD

Chemokine signaling guides regional patterning of the first embryonic artery.
Genes & development. 2009 Oct 1;23(19):2272-7; Authors: Siekmann AF, Standley C, Fogarty KE, Wolfe SA, Lawson ND

A genetic screen for vascular mutants in zebrafish reveals dynamic roles for Vegf/Plcg1 signaling during artery development.
Developmental biology. 2009 May 15;329(2):212-26 Epub 2009 Mar 6; Authors: Covassin LD, Siekmann AF, Kacergis MC, Laver E, Moore JC, Villefranc JA, Weinstein BM, Lawson ND

Modulation of VEGF signalling output by the Notch pathway.
BioEssays : news and reviews in molecular, cellular and developmental biology. 2008 Apr;30(4):303-13; Authors: Siekmann AF, Covassin L, Lawson ND

Notch signalling and the regulation of angiogenesis.
Cell adhesion & migration. 2007 Apr-Jun;1(2):104-6 Epub 2007 Apr 25; Authors: Siekmann AF, Lawson ND

Notch signalling limits angiogenic cell behaviour in developing zebrafish arteries.
Nature. 2007 Feb 15;445(7129):781-4 Epub 2007 Jan 28; Authors: Siekmann AF, Lawson ND

Distinct tissue-specificity of three zebrafish ext1 genes encoding proteoglycan modifying enzymes and their relationship to somitic Sonic hedgehog signaling.
Developmental dynamics : an official publication of the American Association of Anatomists. 2005 Feb;232(2):498-505; Authors: Siekmann AF, Brand M